| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.246514 |
| Chromosome: | chromosome 10 |
| Location: | 2398953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435400 | (1 of 1) PF03398 - Regulator of Vps4 activity in the MVB pathway (Ist1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAACGGTAGGCCAGACGCTAGAGGTAGC |
| Internal bar code: | AGTCACATTTCCTGAGAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 95.9885 |
| LEAP-Seq n confirming: | 335 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTCACCTGGACCTGTTT |
| Suggested primer 2: | CTCGGGGATTGATTTTGCTA |