| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.246944 |
| Chromosome: | chromosome 3 |
| Location: | 4659961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177450 | (1 of 1) IPR003729//IPR022772//IPR024053 - Bifunctional nuclease domain // von Hippel-Lindau disease tumour suppressor, beta/alpha domain // von Hippel-Lindau disease tumour suppressor, beta domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACCCAGATGCCTTGTCGGCGGCTGAGT |
| Internal bar code: | ACTCTGGTCTTACGAGTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTGTGGTGCAGCTTTTG |
| Suggested primer 2: | CGGAGTGATAGTGCTCGTCA |