Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.247480 |
Chromosome: | chromosome 16 |
Location: | 3888403 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g690250 | PUS18 | TruB family RNA pseudouridine synthase; (1 of 1) K03177 - tRNA pseudouridine55 synthase [EC:5.4.99.25] (truB, PUS4, TRUB1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAACCCCCACCCCACCCCATGTAAGGGT |
Internal bar code: | GATACCACCACATTATGAATTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 493 |
LEAP-Seq percent confirming: | 95.9641 |
LEAP-Seq n confirming: | 214 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGAAGCGTACGTTCTTCCC |
Suggested primer 2: | TGCCTAGCGAAGCGTATTTT |