Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.247514 |
Chromosome: | chromosome_13 |
Location: | 2134467 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g577950 | VPS60 | Subunit of the ESCRT-III complex | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACTGGCAAAACGCCCTTTCCCTCCCCCCA |
Internal bar code: | CCAAGGCGATGGGTGTCACATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 437 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 7 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGATACGCAAGCACAAGG |
Suggested primer 2: | GCGAAAGAACAGGTGTGGAT |