Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.248133 |
Chromosome: | chromosome_16 |
Location: | 2594207 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g661626 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCCTGTAGCTCCCCGGCCTCGACTCCTC |
Internal bar code: | TATGCGCTCGCCAGACATGCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 555 |
LEAP-Seq percent confirming: | 93.2308 |
LEAP-Seq n confirming: | 303 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGCTCCTCGGTTTGATAGC |
Suggested primer 2: | TATCGCTGAGCACTCTGCAC |