| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248448 |
| Chromosome: | chromosome 3 |
| Location: | 5086158 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181250 | SNE18,CGLD13 | (1 of 10) PF13460 - NAD(P)H-binding (NAD_binding_10); conserved protein related to nucleoside diphosphate sugar epimerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATGGCTGTATGGTTTCGAACTTGGACC |
| Internal bar code: | TGTCCCAGTAAAGGTTTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.0749 |
| LEAP-Seq n confirming: | 7390 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACACCACTTTGCAGCAG |
| Suggested primer 2: | GCAACAAGTTTTGGCTGTGA |