| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.248969 |
| Chromosome: | chromosome 12 |
| Location: | 3057067 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500500 | SMT1,SMT2,STM1 | (1 of 1) 2.1.1.143 - 24-methylenesterol C-methyltransferase / SMT(2); Sterol-C24-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACCAGGTGAACGGTCTTCGAGCGGTC |
| Internal bar code: | ATGGCGGCTACTTTCGTACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 273 |
| LEAP-Seq percent confirming: | 95.4751 |
| LEAP-Seq n confirming: | 211 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTAATTGCCGTTCAGTG |
| Suggested primer 2: | GTTCTCCTTGAACGGCATGT |