Gene: Cre12.g500500

Overview

Common name:SMT1,SMT2,STM1
Defline:(1 of 1) 2.1.1.143 - 24-methylenesterol C-methyltransferase / SMT(2); Sterol-C24-methyltransferase
Description:Similar to sterol-C24-Methyltransferase from Arabidopsis. Previously annotated as SMT1 but this conflicts with a series of smt mutants identified by Fang and Umen as suppressors of MAT3 size control
Synonyms:g12566.t1,SMT2,SMT1,STM1
Chromosome:chromosome 12

Protein Localization

A plasmid for expressing Cre12.g500500 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g500500-Venus-3xFLAG

Imaged strain: CSI_LW10F10

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

ambiguous

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

Bait ID Bait Name Prey ID Prey Name Reference
Cre02.g097800 HLA3,MRP1 Cre12.g500500 SMT1,SMT2,STM1 Mackinder et al. 2017
Cre04.g223300 CCP1 Cre12.g500500 SMT1,SMT2,STM1 Mackinder et al. 2017

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.013480_1 CLIP2.013480 intron 80 6.1
CLIP2.013480_2 CLIP2.013480 intron 80 6.1
CLIP2.018514_1 CLIP2.018514 intron 80 6.1
CLIP2.018514_2 CLIP2.018514 intron 80 6.1
CLIP2.036139_1 CLIP2.036139 intron 80 6.1
CLIP2.036139_2 CLIP2.036139 intron 80 6.1
CLIP2.048852_1 CLIP2.048852 intron 80 6.1
CLIP2.048852_2 CLIP2.048852 intron 80 6.1
CLIP2.050983_1 CLIP2.050983 intron 80 6.1
CLIP2.050983_2 CLIP2.050983 intron 80 6.1
LMJ.RY0402.045735_1 LMJ.RY0402.045735 3'UTR 73 5.5
LMJ.RY0402.049547_2 LMJ.RY0402.049547 3'UTR 73 5.5
LMJ.RY0402.061387_1 LMJ.RY0402.061387 3'UTR 73 5.5
LMJ.RY0402.064367_1 LMJ.RY0402.064367 intron 95 5.5
LMJ.RY0402.064367_2 LMJ.RY0402.064367 intron 95 5.5
LMJ.RY0402.067661_1 LMJ.RY0402.067661 intron 58 5.5
LMJ.RY0402.067661_2 LMJ.RY0402.067661 intron 58 5.5
LMJ.RY0402.080322_2 LMJ.RY0402.080322 3'UTR 73 5.5
LMJ.RY0402.131327_2 LMJ.RY0402.131327 3'UTR 95 5.5
LMJ.RY0402.158159_1 LMJ.RY0402.158159 3'UTR 73 5.5
LMJ.RY0402.185460_1 LMJ.RY0402.185460 intron 73 5.5
LMJ.RY0402.216720_3 LMJ.RY0402.216720 intron 95 5.5
LMJ.RY0402.216720_4 LMJ.RY0402.216720 intron 95 5.5
LMJ.RY0402.230181_3 LMJ.RY0402.230181 3'UTR 95 5.5
LMJ.RY0402.236200_1 LMJ.RY0402.236200 3'UTR 73 5.5
LMJ.RY0402.248969_1 LMJ.RY0402.248969 intron 95 5.5
LMJ.RY0402.248969_2 LMJ.RY0402.248969 CDS 95 5.5
LMJ.SG0182.019314_1 LMJ.SG0182.019314 MULTIPLE_SPLICE_VARIANTS 75 5.3

Gene Annotations

PFAM:PF08241, PF08498
PANTHER: PTHR10108 PTHR10108:SF930
KEGG_ec:EC:2.1.1.143
KEGG_orthology:K08242
KOG:KOG1269
Gene ontology terms::GO:0006694, GO:0008152, GO:0008168
Best arabidopsis TAIR name:AT1G20330
Best arabidopsis TAIR symbol:CVP1,FRL1,SMT2, SMT3
Best arabidopsis TAIR defline:sterol methyltransferase 2
Phytozome Locus Page

Phenotypes

Mutants in this gene exhibit a phenotype under the following conditions:

Carbon concentrating mechanism, screen 10 of 11 ( 82% confidence (FDR = 0.18))
Carbon concentrating mechanism, screen 11 of 11 ( 86% confidence (FDR = 0.14))
Cisplatin, screen 2 of 4 ( 80% confidence (FDR = 0.2))
Cisplatin, screen 3 of 4 ( 99% confidence (FDR = 0.0094))
Cisplatin, screen 4 of 4 ( 93% confidence (FDR = 0.062))
High temperature, screen 4 of 7 ( 77% confidence (FDR = 0.23))
Mannitol, screen 1 of 1 ( 84% confidence (FDR = 0.16))
Methyl methanesulfonate, screen 3 of 4 ( 75% confidence (FDR = 0.25))
Zeocin, screen 3 of 5 ( 86% confidence (FDR = 0.14))

Note: FDR indicates the likelihood that a particular genotype-phenotype relationship is incorrect. Not all mutants in a particular gene show the same phenotype. Please see the mutant pages for each mutant's phenotypes. Complementation is needed to definitively prove a genotype-phenotype link. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.