| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.216720_1 | Cre02.g099055 | (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 | intron | 73 | |
| LMJ.RY0402.216720_2 | Cre02.g111500 | (1 of 1) PTHR24056//PTHR24056:SF7 - CELL DIVISION PROTEIN KINASE // CYCLIN-DEPENDENT KINASE-LIKE 2 | CDS | 58 | |
| LMJ.RY0402.216720_3 | Cre12.g500500 | SMT1,SMT2,STM1 | (1 of 1) 2.1.1.143 - 24-methylenesterol C-methyltransferase / SMT(2); Sterol-C24-methyltransferase | intron | 95 |
| LMJ.RY0402.216720_4 | Cre12.g500500 | SMT1,SMT2,STM1 | (1 of 1) 2.1.1.143 - 24-methylenesterol C-methyltransferase / SMT(2); Sterol-C24-methyltransferase | intron | 95 |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| Carbon concentrating mechanism, screen 10 of 11 (~40.0% growth defect) |
| Carbon concentrating mechanism, screen 11 of 11 (~30.0% growth defect) |
| Cisplatin, screen 2 of 4 (No phenotype detected) |
| Cisplatin, screen 3 of 4 (~10.0% growth defect) |
| Cisplatin, screen 4 of 4 (~10.0% growth defect) |
| High temperature, screen 4 of 7 (No phenotype detected) |
| Mannitol, screen 1 of 1 (No phenotype detected) |
| Methyl methanesulfonate, screen 3 of 4 (~20.0% growth defect) |
| Zeocin, screen 3 of 5 (No phenotype detected) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.