| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.250114 |
| Chromosome: | chromosome 16 |
| Location: | 7252414 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682699 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCAGGAGCCCGTTGGGCCCGATGATGG |
| Internal bar code: | TCGGTTTCTAGTAATAAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 146 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGTCACTCTTCTGCCG |
| Suggested primer 2: | ACCCATCCCATCCATACTGA |