| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.251204 |
| Chromosome: | chromosome 1 |
| Location: | 345035 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002150 | (1 of 5) IPR023220 - Type IV secretion system, VirB5-domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAAAGCCGAGACGTCAACCAGCTAGACA |
| Internal bar code: | CGAGACACGCTAGCCGCTCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1097 |
| LEAP-Seq percent confirming: | 99.5772 |
| LEAP-Seq n confirming: | 4946 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTCTTTTGATTAGGC |
| Suggested primer 2: | GAGTTGATGGCGGGTACAGT |