| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.251514 |
| Chromosome: | chromosome 6 |
| Location: | 5429599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284200 | LHCBM9 | (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGCAGTGCTTCGTTCCACCACGTCAT |
| Internal bar code: | CCGGGGTTACATGCTAGGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 156 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGTAAGTGCCATTTGT |
| Suggested primer 2: | AACCGAGGTGCTTGAGAGAA |