| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251634 |
| Chromosome: | chromosome 14 |
| Location: | 2272344 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623200 | LAP1 | Leucyl aminopeptidase; (1 of 1) 3.4.11.1//3.4.11.23 - Leucyl aminopeptidase / Peptidase S // PepB aminopeptidase / Peptidase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGGCAACACGGACGCGGAGGGCCGACT |
| Internal bar code: | AGCAAGTCGGCGAGTCCAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 93.7209 |
| LEAP-Seq n confirming: | 403 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCCTTCCCCTCTCTTTG |
| Suggested primer 2: | ACAACACCCGACTGTTCCTT |