| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.251640 |
| Chromosome: | chromosome 1 |
| Location: | 5662343 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040350 | MSH8 | putative MutS type 2 DNA repair protein; (1 of 2) PTHR11361//PTHR11361:SF14 - DNA MISMATCH REPAIR MUTS RELATED PROTEINS // DNA MISMATCH REPAIR PROTEIN MUTS2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTTGGCCTCTCCTCTCAGTTGCCTAC |
| Internal bar code: | CCGGCCGTTTTAAAATGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 99.5868 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAACATCCGTATGCCAA |
| Suggested primer 2: | TAGCAATCATGAGGCAGTCG |