Insertion junction: LMJ.RY0402.252572_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252572
Chromosome:chromosome 16
Location:2582250
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g661550 (1 of 1) PF06941 - 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (NT5C) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAATTCGCTACCTCACAATCCAGGACAG
Internal bar code:AAGACGAATTGACACTTCAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:514
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCTTTGCTGCATTCTGT
Suggested primer 2:TGGAGTTTGGAGTTGGAAGG