Insertion junction: LMJ.RY0402.252629_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252629
Chromosome:chromosome 11
Location:987141
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCAGTGTACGTCCCTGTGACCTATTTC
Internal bar code:CAGTGTGTCCGAGATAAACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:99.4039
LEAP-Seq n confirming:1334
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAAGGTCCTGCTTGCCTG
Suggested primer 2:TTGAAATGCAGCAGGACAAG