| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253005 |
| Chromosome: | chromosome 12 |
| Location: | 8149673 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551200 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACGTGCTTGGTGCGCGTGACGTTTG |
| Internal bar code: | GGAAAGAGTAGGGACCCCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 97.9619 |
| LEAP-Seq n confirming: | 1490 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGCAGCCTCTCTGAAAT |
| Suggested primer 2: | CACGTGTCAAATATGCAGGG |