| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.253212 |
| Chromosome: | chromosome 9 |
| Location: | 1824312 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396000 | NAR5,NRT2C | Nitrate/nitrite transporter; (1 of 4) K02575 - MFS transporter, NNP family, nitrate/nitrite transporter (NRT, narK, nrtP, nasA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGGCGATGAGGTCTGCAGGCGGCGCG |
| Internal bar code: | GGTCAAGTGGTGAATCACGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 98.729 |
| LEAP-Seq n confirming: | 1942 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTGAGTAAGCGGGAAC |
| Suggested primer 2: | GATGGGGGAGGGTTGTAGAT |