Insertion junction: LMJ.RY0402.253293_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.253293
Chromosome:chromosome 3
Location:1431048
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTCCTGTGACCCCGGAGGGCTTGGATAA
Internal bar code:GCGCGAGGAAGGCCCGAGATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:99.5708
LEAP-Seq n confirming:464
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AACACATGAGGAGCTGGGAC
Suggested primer 2:CCGTCAGTGATTTGTGTTGG