| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254043 |
| Chromosome: | chromosome 9 |
| Location: | 3280159 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388060 | VDR1 | violaxanthin de-epoxidase related, chloroplast; (1 of 1) 1.10.99.3 - Transferred entry: 1.23.5.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCGCACCGGGCCGCACCTGCCCGCTC |
| Internal bar code: | CGAATTGGACTGTTGGTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.0991 |
| LEAP-Seq n confirming: | 880 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTAGCATTGTGTCGGGTG |
| Suggested primer 2: | TACTTGGAGCCGCAGTTCTT |