| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254328 |
| Chromosome: | chromosome 5 |
| Location: | 2254224 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234663 | SRTC1,SRTC | (1 of 1) PTHR11085:SF23 - NAD-DEPENDENT HISTONE DEACETYLASE HST4; Sir2-like NADH dependent histone deacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAGACATGTAGGTGCGGCGAGGCACGC |
| Internal bar code: | TTGTATACGCTAAAGAGACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.5493 |
| LEAP-Seq n confirming: | 5080 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCATATTTAGCGGCTTG |
| Suggested primer 2: | TCCTACCATCCCATTTCAGC |