| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254353 |
| Chromosome: | chromosome 10 |
| Location: | 1281600 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g427150 | SPL16 | (1 of 1) K12870 - pre-mRNA-splicing factor ISY1 (ISY1); Pre-mRNA splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATCCTTGAGGGAGGGGTTCCTTTCT |
| Internal bar code: | CTAACGCGATGGGCGATGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGATGAGGGGTGTTACG |
| Suggested primer 2: | CTGACCAGCTCCTTCCACTC |