| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.254413 |
| Chromosome: | chromosome 16 |
| Location: | 863401 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647950 | (1 of 33) IPR015797 - NUDIX hydrolase domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCTTGAAGTGACAACCCGCTTGGAAGC |
| Internal bar code: | TAGTTGCGCTAAGGTCACGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 93.3484 |
| LEAP-Seq n confirming: | 10357 |
| LEAP-Seq n nonconfirming: | 738 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGTGCTTCTTGGGGT |
| Suggested primer 2: | CAACCGTGCTATGTTGTTGG |