| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.254450 |
| Chromosome: | chromosome 6 |
| Location: | 3401168 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278086 | (1 of 1) PTHR11803//PTHR11803:SF17 - TRANSLATION INITIATION INHIBITOR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTATGCGATGCAGTGTGCGTGTGTTAAAGA |
| Internal bar code: | CGTAAATTTTCAGGAATCGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 69.0962 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 106 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGAAAGTTGGGTGGGAG |
| Suggested primer 2: | CATGCCCTCCATCTTCCTAA |