| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.254881 |
| Chromosome: | chromosome 6 |
| Location: | 6168269 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290400 | HDA5 | (1 of 1) PTHR10625:SF104 - HISTONE DEACETYLASE 6; RPD3/HDA1 type histone deacetylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGAAGGATCCCGGAGACAGATACGTT |
| Internal bar code: | AGTGCACCCCACGAGGTAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 94.6381 |
| LEAP-Seq n confirming: | 353 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTTCCTTAGACTCCAAC |
| Suggested primer 2: | CCAAGGGCAGGAAGATTACA |