| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255109 |
| Chromosome: | chromosome 12 |
| Location: | 5863426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCGACGTCGGTGTGCGCCGCCCAGTC |
| Internal bar code: | GCCAGCGGGATATTCAAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 428 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCCTCGCTCTGGTCTG |
| Suggested primer 2: | CCAACAACCTCCACACTCCT |