Insertion junction: LMJ.RY0402.255584_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.255584
Chromosome:chromosome 5
Location:2507730
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g235750 MSH6 DNA mismatch repair protein, MutS homolog; (1 of 1) K08737 - DNA mismatch repair protein MSH6 (MSH6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTAGAAGGTCCACCCAGTCACAAAATGGA
Internal bar code:CCTCAGTCCGCCCGAATTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGATTAGCTTGGGAAACGG
Suggested primer 2:ACATTCCGAGCTATTGACGG