| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255998 |
| Chromosome: | chromosome_4 |
| Location: | 2957533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224800 | VAMP74,VAM4 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTAAACCCATCGGCACACCGTAGACAGTCC |
| Internal bar code: | CGCTGGTGCAGAGGGACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.1342 |
| LEAP-Seq n confirming: | 458 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTGGTTGCATGTTTGC |
| Suggested primer 2: | ACAAGGTTTTGTCCGTCAGG |