| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256000 |
| Chromosome: | chromosome_13 |
| Location: | 1663509 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g574000 | CLV1,GEF1 | Voltage-gated chloride channel | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCATTTGTTCAGCCTGCGTGCATGTGTT |
| Internal bar code: | CAACTGGTTCTCACGGGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 99.505 |
| LEAP-Seq n confirming: | 201 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGGAAGGCACGCACTTTA |
| Suggested primer 2: | GTGGTGTGATGTTCGTGAGG |