Insertion junction: LMJ.RY0402.256291_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256291
Chromosome:chromosome 6
Location:5550054
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g285250 LHCBM6 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chloropyll a/b binding protein of LHCII type I, chloroplast precursor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGAGAAGCACTACTGCCGTGCGCTGAA
Internal bar code:TTGTCGATCCCCACGCCCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:26
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:142
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCCATTGATACCGTTCCA
Suggested primer 2:CTACTCTGAGAACGCGACCC