| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256638 |
| Chromosome: | chromosome 12 |
| Location: | 8179071 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550900 | SSD3 | Sterol sensing domain protein; (1 of 1) K12385 - Niemann-Pick C1 protein (NPC1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGTTGAGGAATGCAGATTGCCCGCCCG |
| Internal bar code: | TTGGATTTCACGTCGGCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.8188 |
| LEAP-Seq n confirming: | 1102 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGTAGGTGCGGAAGCTG |
| Suggested primer 2: | GCACACAAACGCTGTCAAGT |