Insertion junction: LMJ.RY0402.256838_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.256838
Chromosome:chromosome 11
Location:3152474
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g479300 SRH20 SNF2-related DNA/RNA helicase; (1 of 1) K15083 - DNA repair protein RAD16 (RAD16) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCATCACCGCTCCCCGAGTCACCCTCGT
Internal bar code:GCCGAGAATCGGGGGTGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACCCCATCAAC
Suggested primer 2:ACACACACACACACACACCG