| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.257212 |
| Chromosome: | chromosome 9 |
| Location: | 2051214 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394150 | RAA1,OPR40 | psaA mRNA trans-splicing factor; (1 of 3) PF06743 - FAST kinase-like protein, subdomain 1 (FAST_1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGCGCACAAACGAAGCACCATGTTGCT |
| Internal bar code: | AACCGACCCATGCCCCTAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 91.2021 |
| LEAP-Seq n confirming: | 1047 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGTTCAGTTTTCTTCGC |
| Suggested primer 2: | AGCTGCGTCTTGTTGGAAGT |