Insertion junction: LMJ.SG0182.000676_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.000676
Chromosome:chromosome 12
Location:6884557
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre12.g560900 CPLD35,AOF7 Flavin-containing amine oxidase. Conserved in plant lineage and Diatoms; (1 of 2) 1.3.5.5 - 15-cis-phytoene desaturase / Plant-type phytoene desaturase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGCGAGGACGCCGGCATC
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:695
LEAP-Seq percent confirming:98.7179
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGGGAATTAGATGCACCC
Suggested primer 2:CGACCACCAACACATCACTC