Insertion junction: LMJ.SG0182.004226_1

Overview

Insertion cassette:pMJ013b
Side of cassette:5'
Strand:-
Strain:LMJ.SG0182.004226
Chromosome:chromosome 4
Location:1262999
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre04.g215050 CHYB,CHYB1,CBH1 Beta-carotene hydroxylase, putative chloroplast precursor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTAGACTCCTTGCAACCA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:632
LEAP-Seq percent confirming:95.9184
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTCGGATGAGAGCATT
Suggested primer 2:AGACAGAGCACACACGCAAC