| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.019047 |
| Chromosome: | chromosome_4 |
| Location: | 2955626 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224800 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCAGCAGGATGACCGCAAGGG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1374 |
| LEAP-Seq percent confirming: | 94.9468 |
| LEAP-Seq n confirming: | 357 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTGGGGTGAGGCGTAAA |
| Suggested primer 2: | CTATCTGTGTGCGTGTGCCT |