| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.SG0182.019236 |
| Chromosome: | chromosome 10 |
| Location: | 2910682 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440200 | SMC5B | (1 of 2) PTHR19306:SF1 - STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5; Structural Maintenance of Chromosomes protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCACGCATAGAAAGCAAT |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1213 |
| LEAP-Seq percent confirming: | 94.9602 |
| LEAP-Seq n confirming: | 358 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGGAGTGGAAAAATGGAA |
| Suggested primer 2: | CCACGGCTCAGTCCATAACT |