Insertion junction: LMJ.SG0182.019293_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:+
Strain:LMJ.SG0182.019293
Chromosome:chromosome 3
Location:6352007
Confidence (%):75
Locus systematic id Locus common name Defline Feature
Cre03.g193800 TSN1 Aspartyl-tRNA synthetase; (1 of 1) 6.1.1.22 - Asparagine--tRNA ligase / Asparaginyl-tRNA synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGTGCGCACGATCGAGAAA
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:872
LEAP-Seq percent confirming:98.5981
LEAP-Seq n confirming:211
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCGTCTTTTGGGTGTGGT
Suggested primer 2:CTTCTGTGCATTGCCACTGT