Insertion cassette: | pMJ013b |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.SG0182.019478 |
Chromosome: | chromosome_7 |
Location: | 1238701 |
Confidence (%): | 75 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGCTTCAAGGCCACCTGCAG |
Internal bar code: | |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1059 |
LEAP-Seq percent confirming: | 96.6523 |
LEAP-Seq n confirming: | 895 |
LEAP-Seq n nonconfirming: | 31 |
LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGAGCGCATTTCCTAAAGC |
Suggested primer 2: | ACAGGAGCAGAGAGGCAAAA |