| Insertion cassette: | pMJ013b |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.SG0182.020074 |
| Chromosome: | chromosome_12 |
| Location: | 5546201 |
| Confidence (%): | 75 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g531400 | NPH4 | Nephrocystin-4-like protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACCAGCGACATGGCCTACCTG |
| Internal bar code: | |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1222 |
| LEAP-Seq percent confirming: | 99.1667 |
| LEAP-Seq n confirming: | 357 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCTGAAGGACGTGAGT |
| Suggested primer 2: | GCACTAGTGAGCCCTTGGAG |