Insertion junction: LMJ.SG0182.020278_1

Overview

Insertion cassette:pMJ013b
Side of cassette:3'
Strand:-
Strain:LMJ.SG0182.020278
Chromosome:chromosome_12
Location:1182653
Confidence (%):75
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g489900 CYG54 Adenylate/guanylate cyclase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTGGCACGCCCTTGGGCTTGT
Internal bar code:

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGAAGCAAGCATAGTCGG
Suggested primer 2:TTTTTGGCGGTCATCTTTTC