Mutant strain: LMJ.RY0402.104890

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.104890_1 Cre02.g078804 3'UTR 73
LMJ.RY0402.104890_2 Cre02.g078831 (1 of 1) IPR002059//IPR012340 - Cold-shock protein, DNA-binding // Nucleic acid-binding, OB-fold 5'UTR 73
LMJ.RY0402.104890_3 Cre09.g387400 POLZ1 DNA polymerase zeta; (1 of 1) K02350 - DNA polymerase zeta (POLZ1, rev3) intron 58

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Bleomycin sulfate, screen 1 of 2 (~40.0% growth defect)
Bleomycin sulfate, screen 2 of 2 (~20.0% growth defect)
Cisplatin, screen 2 of 4 (~40.0% growth defect)
Cisplatin, screen 3 of 4 (~70.0% growth defect)
Cisplatin, screen 4 of 4 (~40.0% growth defect)
Methyl methanesulfonate, screen 4 of 4 (~10.0% improved growth)
Mitomycin C, screen 2 of 4 (~90.0% growth defect)
Mitomycin C, screen 3 of 4 (No growth detected)
Mitomycin C, screen 4 of 4 (~90.0% growth defect)
UV radiation, screen 2 of 2 (No growth detected)
Zeocin, screen 2 of 5 (~20.0% growth defect)
Zeocin, screen 3 of 5 (No phenotype detected)
Zeocin, screen 4 of 5 (~10.0% growth defect)
Zeocin, screen 5 of 5 (No phenotype detected)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.