Mutant strain: LMJ.RY0402.173847

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.173847_1 Cre05.g232550 GPM4,PGM4,CA1P1 (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase CDS 95
LMJ.RY0402.173847_2 Cre05.g232550 GPM4,PGM4,CA1P1 (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase CDS 95
LMJ.RY0402.173847_3 Cre16.g650900 (1 of 1) IPR000104//IPR001965//IPR011011//IPR017956 - Antifreeze protein, type I // Zinc finger, PHD-type // Zinc finger, FYVE/PHD-type // AT hook, DNA-binding motif CDS 95
LMJ.RY0402.173847_4 Cre16.g650900 (1 of 1) IPR000104//IPR001965//IPR011011//IPR017956 - Antifreeze protein, type I // Zinc finger, PHD-type // Zinc finger, FYVE/PHD-type // AT hook, DNA-binding motif CDS 95
LMJ.RY0402.173847_5 Cre16.g650900 (1 of 1) IPR000104//IPR001965//IPR011011//IPR017956 - Antifreeze protein, type I // Zinc finger, PHD-type // Zinc finger, FYVE/PHD-type // AT hook, DNA-binding motif CDS 58

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Carbon concentrating mechanism, screen 3 of 11 (~30.0% growth defect)
Carbon concentrating mechanism, screen 4 of 11 (~20.0% growth defect)
Carbon concentrating mechanism, screen 5 of 11 (~10.0% growth defect)
Carbon concentrating mechanism, screen 10 of 11 (~10.0% growth defect)
Carbon concentrating mechanism, screen 11 of 11 (No phenotype detected)
High temperature, high CO2 in minimal media, screen 2 of 2 (~10.0% improved growth)
Hydroxyurea, screen 2 of 2 (~30.0% improved growth)
LAT027F04, screen 1 of 1 (~20.0% growth defect)
LAT030E11, screen 1 of 1 (No phenotype detected)
LAT033G04, screen 1 of 1 (~20.0% growth defect)
LAT041D11, screen 1 of 1 (~20.0% improved growth)
LAT042F07, screen 1 of 1 (No phenotype detected)
Photosynthesis, screen 4 of 5 (~20.0% growth defect)
Photosynthesis, screen 5 of 5 (~10.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.