Gene: Cre12.g510800
Overview
| Common name: | CHLI2 |
| Defline: | (1 of 2) PTHR32039//PTHR32039:SF9 - FAMILY NOT NAMED // MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC; Magnesium chelatase subunit I, isoform 2 with histidine kinase activity |
| Description: | Magnesium-chelatase subunit chlI, chloroplast precursor (Mg-protoporphyrin IX chelatase); Orthologous to the bacterial magnesium chelatase subunits BchI, and other plant magnesium chelatase subunits ChlI [PMID: 9359397]; Activates and phosphorylates a histidine on CHLD to activate magnesium chelatase activity and is unable to replace CHLI1 subunit in the magnesium chelatase reaction [PMID: 28487379]; chloroplast targeting signal peptide predicted 1-37 by ChloroP. |
| Synonyms: | g12332.t1,CHLI2,Cre12.g510800.t1.1 |
| Chromosome: | chromosome 12 |
Protein Localization
A plasmid for expressing Cre12.g510800 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g510800-Venus-3xFLAG
Imaged strain: CSI_LW06G12
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
chloroplast/homogeneous/pyrenoid depleted
shapes in cytoplasm/uncategorized shapes in cytoplasm
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.001967_1 | CLIP2.001967 | intron | 80 | 6.1 |
| CLIP2.001967_2 | CLIP2.001967 | intron | 80 | 6.1 |
| CLIP2.002261_2 | CLIP2.002261 | intron | 80 | 6.1 |
| CLIP2.002261_3 | CLIP2.002261 | intron | 80 | 6.1 |
| CLIP2.004497_1 | CLIP2.004497 | 3'UTR | 80 | 6.1 |
| CLIP2.004497_2 | CLIP2.004497 | 3'UTR | 80 | 6.1 |
| CLIP2.006303_1 | CLIP2.006303 | CDS | 80 | 6.1 |
| CLIP2.006303_2 | CLIP2.006303 | CDS | 80 | 6.1 |
| CLIP2.007378_1 | CLIP2.007378 | CDS | 80 | 6.1 |
| CLIP2.007580_1 | CLIP2.007580 | intron | 80 | 6.1 |
| CLIP2.007580_2 | CLIP2.007580 | intron | 80 | 6.1 |
| LMJ.RY0402.140257_1 | LMJ.RY0402.140257 | 3'UTR | 73 | 5.5 |
| LMJ.RY0402.159788_1 | LMJ.RY0402.159788 | intron | 73 | 5.5 |
| LMJ.RY0402.198787_1 | LMJ.RY0402.198787 | CDS | 58 | 5.5 |
Gene Annotations
| PFAM: | PF01078 |
| PANTHER: | PTHR32039 PTHR32039:SF9 |
| KEGG_ec: | EC:6.6.1.1 |
| KEGG_orthology: | |
| KOG: | |
| Gene ontology terms:: | GO:0005524, GO:0006779, GO:0015979, GO:0015995, GO:0016851 |
| Best arabidopsis TAIR name: | AT4G18480.1 |
| Best arabidopsis TAIR symbol: | CH-42,CH42,CHL11,CHLI-1,CHLI1 |
| Best arabidopsis TAIR defline: | P-loop containing nucleoside triphosphate hydrolases superfamily |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.