Insertion junction: CLIP2.000679_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000679
Chromosome:chromosome 12
Location:4934846
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g524300 CGL71,PYG7 (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase; Pale yellow green 7, a PSI assembly factor 3'UTR
Cre12.g524350 HUS1 DNA damage checkpoint protein; (1 of 1) K10903 - HUS1 checkpoint protein (HUS1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCATGCACTAGGCGAAGATCCACGCAACAGGCGCGGCTCCACGCCGCA
Internal bar code:GAAATGTTTTGGCTTGTGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1397
LEAP-Seq percent confirming:94.5946
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCGTTGCACTTGCCAAAC
Suggested primer 2:CCCTAAAATTGGCGCCGATG