Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.000679 |
Chromosome: | chromosome 12 |
Location: | 4934846 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g524300 | CGL71,PYG7 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase; Pale yellow green 7, a PSI assembly factor | 3'UTR |
Cre12.g524350 | HUS1 | DNA damage checkpoint protein; (1 of 1) K10903 - HUS1 checkpoint protein (HUS1) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCATGCACTAGGCGAAGATCCACGCAACAGGCGCGGCTCCACGCCGCA |
Internal bar code: | GAAATGTTTTGGCTTGTGGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1397 |
LEAP-Seq percent confirming: | 94.5946 |
LEAP-Seq n confirming: | 35 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCGTTGCACTTGCCAAAC |
Suggested primer 2: | CCCTAAAATTGGCGCCGATG |