Insertion junction: CLIP2.006006_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.006006
Chromosome:chromosome 3
Location:6503275
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g193800 TSN1 Aspartyl-tRNA synthetase; (1 of 1) 6.1.1.22 - Asparagine--tRNA ligase / Asparaginyl-tRNA synthetase 5'UTR
Cre03.g193850 SUCLG1,SCLA1,TEF25,SCL1 (1 of 1) K01899 - succinyl-CoA synthetase alpha subunit (LSC1); Succinyl-CoA ligase alpha chain 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACAGGCGATTTGCTGATGCGTTTGACCTTCACCTTGCTGTCACGTTT
Internal bar code:ACACTGAGGTCCCCTTACGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:741
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTACACAGTGTCCAGCTGCT
Suggested primer 2:CAACGAACACAGCTGGGTTG