| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.006643 |
| Chromosome: | chromosome 12 |
| Location: | 456170 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494750 | PRPS20 | Chloroplast ribosomal protein S20; (1 of 1) K02968 - small subunit ribosomal protein S20 (RP-S20, rpsT) | 3'UTR |
| Cre12.g494800 | DII1,IDA-IC28,IDA4,p28IDA4 | (1 of 1) K10410 - dynein light intermediate chain, axonemal (DNALI); Flagellar inner arm dynein light chain p28 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTAGCGTGAGCAGAACGCATACGCAACCTTCCGACCTCGACTTAACG |
| Internal bar code: | AACTGGTGATAGGGCACCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2688 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATTGGGGGCTTGGCTTG |
| Suggested primer 2: | CGGGATAGGGGTGTTCGATG |