| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025228 |
| Chromosome: | chromosome 2 |
| Location: | 4773562 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108700 | (1 of 4) 2.7.10.2//2.7.11.1//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase | 5'UTR_intron | |
| Cre02.g108750 | (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGGAAGCGCGGGGCAAGGCAAGCGTGTCCATCCCTGAAACTATCCCC |
| Internal bar code: | GGCCTGGACGCACGGCTTGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2553 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAGGAGAGCCAGAAGGG |
| Suggested primer 2: | GTTGGTGCCTTTGACAGCTG |