Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.168619 |
Chromosome: | chromosome_12 |
Location: | 8333079 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g549550 | RSP19,TUB2 | Beta tubulin 2 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGAGCTAATCCGACGAGATGAATGTCCATG |
Internal bar code: | ACGGGCATGTCGAGGAAACGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 213 |
LEAP-Seq percent confirming: | 99.7135 |
LEAP-Seq n confirming: | 348 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGACTGGTACGTGAATGA |
Suggested primer 2: | GAGTTCACTGAGGCCGAGTC |