| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.225746 |
| Chromosome: | chromosome 5 |
| Location: | 2501229 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235700 | (1 of 2) K12382 - saposin (PSAP, SGP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATGTGGACCGCGGCGACCAAGTTTGCG |
| Internal bar code: | TCCGTCGGGCACGAGCGTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 98.7982 |
| LEAP-Seq n confirming: | 1562 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAATCAGGAGTCGGCTA |
| Suggested primer 2: | TTAAATCCTATTCCGCCACG |